Hierarchical Transcriptional Control of the LuxR Quorum-Sensing Regulon of Vibrio harveyi.

ABSTRACT: In vibrios, quorum sensing controls hundreds of genes that are required for cell density-specific behaviors including bioluminescence, biofilm formation, competence, secretion, and swarming motility. The central transcription factor in the quorum-sensing pathway is LuxR/HapR, which directly regulates ∼100 genes in the >400-gene regulon of Vibrio harveyi Among these directly controlled genes are 15 transcription factors, which we predicted would comprise the second tier in the hierarchy of the LuxR regulon. We confirmed that LuxR binds to the promoters of these genes in vitro and quantified the extent of LuxR activation or repression of transcript levels. Transcriptome sequencing (RNA-seq) indicates that most of these transcriptional regulators control only a few genes, with t

SUBMITTER: Chaparian RR 

PROVIDER: S-EPMC7317047 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature