Chimeric Interaction of Nitrogenase-Like Reductases with the MoFe Protein of Nitrogenase.

ABSTRACT: The engineering of transgenic organisms with the ability to fix nitrogen is an attractive possibility. However, oxygen sensitivity of nitrogenase, mainly conferred by the reductase component (NifH)₂ , is an imminent problem. Nitrogenase-like enzymes involved in coenzyme F₄₃₀ and chlorophyll biosynthesis utilize the highly homologous reductases (CfbC)₂ and (ChlL)₂ , respectively. Chimeric protein-protein interactions of these reductases with the catalytic component of nitrogenase (MoFe protein) did not support nitrogenase activity. Nucleotide-dependent association and dissociation of these complexes was investigated, but (CfbC)₂ and wild-type (ChlL)₂ showed no modulation of the binding affinity. By contrast, the interaction b

SUBMITTER: Jasper J 

PROVIDER: S-EPMC7317204 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature