Nanoscale regulation of L-type calcium channels differentiates between ischemic and dilated cardiomyopathies.

ABSTRACT:

Background

Subcellular localization and function of L-type calcium channels (LTCCs) play an important role in regulating contraction of cardiomyocytes. Understanding how this is affected by the disruption of transverse tubules during heart failure could lead to new insights into the disease.

Methods

Cardiomyocytes were isolated from healthy donor hearts, as well as from patients with cardiomyopathies and with left ventricular assist devices. Scanning ion conductance and confocal microscopy was used to study membrane structures in the cells. Super-resolution scanning patch-clamp was used to examine LTCC function in different microdomains. Computational modeling predicted the impact of these changes to arrhythmogenesis at the whole-heart level.

Findings

We showed that l

SUBMITTER: Sanchez-Alonso JL 

PROVIDER: S-EPMC7317229 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature