Extremely low viral reservoir in treated chronically HIV-1-infected individuals.

ABSTRACT:

Background

Small viral reservoirs are found predominantly in HIV-1 controllers and individuals treated during acute/early HIV-1 infection. However, other HIV⁺ individuals could naturally also harbour low viral reservoirs.

Methods

We screened 451 HIV-1-infected treated-individuals with suppressed plasma viremia for at least 3 years and stored cryopreserved peripheral blood mononuclear cells (PBMCs). Total HIV-DNA was analysed in PBMCs with ddPCR. Individuals with <50 HIV-DNA copies/10⁶ PBMCs constitute the 'Low Viral Reservoir Treated' cohort (LoViReT). Longitudinal samples were obtained from 12 chronically treated LoViReT and compared to 13 controls (>50 HIV-DNA copies/10⁶ PBMCs) to analyse total HIV-DNA, T-cell and NK-cell populations, HIV-1

SUBMITTER: Galvez C 

PROVIDER: S-EPMC7317241 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature