Lolium perenne apoplast metabolomics for identification of novel metabolites produced by the symbiotic fungus Epichloe festucae.

ABSTRACT: Epichloë festucae is an endophytic fungus that forms a symbiotic association with Lolium perenne. Here we analysed how the metabolome of the ryegrass apoplast changed upon infection of this host with sexual and asexual isolates of E. festucae. A metabolite fingerprinting approach was used to analyse the metabolite composition of apoplastic wash fluid from uninfected and infected L. perenne. Metabolites enriched or depleted in one or both of these treatments were identified using a set of interactive tools. A genetic approach in combination with tandem MS was used to identify a novel product of a secondary metabolite gene cluster. Metabolites likely to be present in the apoplast were identified using MarVis in combination with the BioCyc and KEGG databases, and an in-house Epichloë metaboli

SUBMITTER: Green KA 

PROVIDER: S-EPMC7317419 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature