Dysbiosis of the Oral Ecosystem in Severe Congenital Neutropenia Patients.

ABSTRACT:

Purpose

To decipher the underlying immunological mechanisms in predisposition to oral microbial dysbiosis in severe congenital neutropenia (SCN) patients.

Experimental design

Ten SCN patients (5-23 years old) and 12 healthy controls (5-22 years old) are periodontally examined and provided saliva, subgingival plaque, and gingival crevicular fluid (GCF) samples. The SCN patients received oral hygiene therapy and are re-evaluated after 6 months. Antimicrobial peptides HPN1-3 and LL-37 are assessed in saliva by ELISA. Concentration of 30 cytokines is measured in saliva and GCF by human 30-plex panel, while bacterial profiles of saliva and subgingival plaque are assessed using 16S rDNA amplicon sequencing.

Results

There is no significant difference in salivary HPN1-3 and L

SUBMITTER: Zaura E 

PROVIDER: S-EPMC7317524 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature