A Trifunctional Linker for Palmitoylation and Peptide and Protein Localization in Biological Membranes.

ABSTRACT: Attachment of lipophilic groups is an important post-translational modification of proteins, which involves the coupling of one or more anchors such as fatty acids, isoprenoids, phospholipids, or glycosylphosphatidyl inositols. To study its impact on the membrane partitioning of hydrophobic peptides or proteins, we designed a tyrosine-based trifunctional linker. The linker allows the facile incorporation of two different functionalities at a cysteine residue in a single step. We determined the effect of the lipid modification on the membrane partitioning of the synthetic α-helical model peptide WALP with or without here and in all cases below; palmitoyl groups in giant unilamellar vesicles that contain a liquid-ordered (L_o ) and liquid-disordered (L_d ) phase. Introduc

SUBMITTER: Syga L 

PROVIDER: S-EPMC7317724 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature