A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation.

ABSTRACT: Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.

SUBMITTER: Kielkowski P 

PROVIDER: S-EPMC7317759 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature