Structure-Guided Design of a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine.

ABSTRACT: Identification of glycan functional epitopes is of paramount importance for rational design of glycoconjugate vaccines. We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb. Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide. Here, starting from the X-ray crystallographic structure

SUBMITTER: Oldrini D 

PROVIDER: S-EPMC7317837 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature