Opsin 3 is a key regulator of ultraviolet A-induced photoageing in human dermal fibroblast cells.

ABSTRACT:

Background

Chronic exposure to ultraviolet (UV) radiation (mainly UVA) induces a sustained increase of matrix metalloproteinases (MMPs), especially MMP1, MMP2, MMP3 and MMP9 in human skin fibroblasts. MMPs can lead to the degradation of fibrous connective tissue, which is the main cause of skin photoageing. The molecular mechanisms through which fibroblasts sense UVA and trigger the cell signalling pathways involved in the upregulation of MMPs have not been well elucidated.

Objectives

Here, we investigated the function and potential mechanisms of photoageing of opsin (OPN)3 in normal human dermal fibroblasts (NHDFs).

Methods

Real-time polymerase chain reaction and Western blot analysis were used to analyse the expression levels of OPN3 in NHDFs and photoageing models.

SUBMITTER: Lan Y 

PROVIDER: S-EPMC7318274 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature