Proteoglycan synthesis rate as a novel method to measure blood-induced cartilage degeneration in non-haemophilic and haemophilic rats.

ABSTRACT:

Introduction

Haemophilic animal models are used to study blood-induced cartilage damage, but quantitative and sensitive outcome measures are needed.

Aim

To develop a novel quantitative method for detecting early cartilage degeneration in a haemophilic rat model of blood-induced joint damage.

Methods

The ³⁵ Sulphate incorporation (³⁵ SO₄ ^2- assay) was applied to tibial and patellar cartilage of wild-type rats to quantify baseline proteoglycan synthesis and to evaluate the effect of 4-day blood exposure in vitro. Next, haemarthrosis was induced in 39 FVIII-deficient rats and characterized by changes in knee joint diameter and development of bone pathology (using micro-CT). Four- and 16-day posthaemarthrosis proteoglycan synthe

SUBMITTER: Pulles AE 

PROVIDER: S-EPMC7318356 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature