Metabolic regulation of hepatic PNPLA3 expression and severity of liver fibrosis in patients with NASH.

ABSTRACT:

Background and aims

The genetic PNPLA3 polymorphism I148M has been extensively associated with higher risk for development and progression of NAFLD towards NASH.

Methods

PNPLA3 and α-SMA expression were quantified in liver biopsies collected from NASH patients (n = 26) with different fibrosis stages and PNPLA3 genotypes. To study the potential mechanisms driving PNPLA3 expression during NASH progression towards fibrosis, hepatocytes and hepatic stellate cells (HSCs) were cultivated in low and high glucose medium. Moreover, hepatocytes were treated with increasing concentrations of palmitic acid alone or in combination with glucose. Conditioned media were collected from challenged hepatocytes to stimulate HSCs.

Results

Tissue expression of PNPLA3 was significantly enha

SUBMITTER: Bruschi FV 

PROVIDER: S-EPMC7318357 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature