Lung delivery of MSCs expressing anti-cancer protein TRAIL visualised with ⁸⁹Zr-oxine PET-CT.

ABSTRACT:

Background

MSCTRAIL is a cell-based therapy consisting of human allogeneic umbilical cord-derived MSCs genetically modified to express the anti-cancer protein TRAIL. Though cell-based therapies are typically designed with a target tissue in mind, delivery is rarely assessed due to a lack of translatable non-invasive imaging approaches. In this preclinical study, we demonstrate ⁸⁹Zr-oxine labelling and PET-CT imaging as a potential clinical solution for non-invasively tracking MSCTRAIL biodistribution. Future implementation of this technique should improve our understanding of MSCTRAIL during its evaluation as a therapy for metastatic lung adenocarcinoma.

Methods

MSCTRAIL were radiolabelled with ⁸⁹Zr-oxine and assayed for viability, phenotype, and therap

SUBMITTER: Patrick PS 

PROVIDER: S-EPMC7318529 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature