Correction of z-motion artefacts to allow population imaging of synaptic activity in behaving mice.

ABSTRACT:

Key points

Motion artefacts associated with motor behaviour are an inevitable problem of multiphoton imaging in awake behaving animals, particularly when imaging synapses. Correction of axial motion artefacts usually requires volumetric imaging resulting in slower rates of acquisition. We describe a method to correct z-motion artefacts that is easy to implement and allows population imaging of synaptic activity while scanning a single plane in a standard multiphoton microscope. The method uses a reference volume acquired in two colour channels - an activity reporter and an anatomical marker of blood vessels. The procedure estimates the z-displacement in every frame and applies an intensity correction in which the z intensity profile for each synapse is modelled as a Moffat function

SUBMITTER: Ryan TM 

PROVIDER: S-EPMC7318612 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature