Resolving Binding Events on the Multifunctional Human Serum Albumin.

ABSTRACT: Physiological processes rely on initial recognition events between cellular components and other molecules or modalities. Biomolecules can have multiple sites or mode of interaction with other molecular entities, so that a resolution of the individual binding events in terms of spatial localization as well as association and dissociation kinetics is required for a meaningful description. Here we describe a trichromatic fluorescent binding- and displacement assay for simultaneous monitoring of three individual binding sites in the important transporter and binding protein human serum albumin. Independent investigations of binding events by X-ray crystallography and time-resolved dynamics measurements (switchSENSE technology) confirm the validity of the assay, the localization of binding sit

SUBMITTER: Wenskowsky L 

PROVIDER: S-EPMC7318646 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature