Genome-Scale Metabolic Model Driven Design of a Defined Medium for Campylobacter jejuni M1cam.

ABSTRACT: Campylobacter jejuni, the most frequent cause of food-borne bacterial gastroenteritis, is a fastidious organism when grown in the laboratory. Oxygen is required for growth, despite the presence of the metabolic mechanism for anaerobic respiration. Amino acid auxotrophies are variably reported and energy metabolism can occur through several electron donor/acceptor combinations. Overall, the picture is one of a flexible, but vulnerable metabolism. To understand Campylobacter metabolism, we have constructed a fully curated, metabolic model for the reference organism M1 (our variant is M1cam) and validated it through laboratory experiments. Our results show that M1cam is auxotrophic for methionine, niacinamide, and pantothenate. There are complete biosynthesis pathways for all am

SUBMITTER: Tejera N 

PROVIDER: S-EPMC7318876 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature