Prior evolution in stochastic versus constant temperatures affects RNA virus evolvability at a thermal extreme.

ABSTRACT: It is unclear how historical adaptation versus maladaptation in a prior environment affects population evolvability in a novel habitat. Prior work showed that vesicular stomatitis virus (VSV) populations evolved at constant 37°C improved in cellular infection at both 29°C and 37°C; in contrast, those evolved under random changing temperatures between 29°C and 37°C failed to improve. Here, we tested whether prior evolution affected the rate of adaptation at the thermal-niche edge: 40°C. After 40 virus generations in the new environment, we observed that populations historically evolved at random temperatures showed greater adaptability. Deep sequencing revealed that most of the newly evolved mutations were de novo. Also, two novel evolved mutations in the VSV glycoprotein and replicase gene

SUBMITTER: Gloria-Soria A 

PROVIDER: S-EPMC7319105 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature