Prediction of synonymous corrections by the BE-FF computational tool expands the targeting scope of base editing.

ABSTRACT: Base editing is a genome-editing approach that employs the CRISPR/Cas system to precisely install point mutations within the genome. A deaminase enzyme is fused to a deactivated Cas and enables transition conversions. The diversified repertoire of base editors provides a wide range of base editing possibilities. However, existing base editors cannot induce transversion substitutions and activate only within a specified region relative to the binding site, thus, they cannot precisely correct every point mutation. Here, we present BE-FF (Base Editors Functional Finder), a novel computational tool that identifies suitable base editors to correct the translated sequence erred by a point mutation. When a precise correction is impossible, BE-FF aims to mutate bystander nucleotides in order to in

SUBMITTER: Rabinowitz R 

PROVIDER: S-EPMC7319459 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature