Synaptotagmin-1 is the Ca²⁺ sensor for fast striatal dopamine release.

ABSTRACT: Dopamine powerfully controls neural circuits through neuromodulation. In the vertebrate striatum, dopamine adjusts cellular functions to regulate behaviors across broad time scales, but how the dopamine secretory system is built to support fast and slow neuromodulation is not known. Here, we set out to identify Ca²⁺-triggering mechanisms for dopamine release. We find that synchronous dopamine secretion is abolished in acute brain slices of conditional knockout mice in which Synaptotagmin-1 is removed from dopamine neurons. This indicates that Synaptotagmin-1 is the Ca²⁺ sensor for fast dopamine release. Remarkably, dopamine release induced by strong depolarization and asynchronous release during stimulus trains are unaffected by Synaptotagmin-1 knockout. Microdialysis

SUBMITTER: Banerjee A 

PROVIDER: S-EPMC7319770 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature