Involvement of the M-CSF/IL-34/CSF-1R pathway in malignant pleural mesothelioma.

ABSTRACT:

Background

Malignant pleural mesothelioma (MPM) is a rare and aggressive cancer related to asbestos exposure. The tumor microenvironment content, particularly the presence of macrophages, was described as crucial for the development of the disease. This work aimed at studying the involvement of the M-CSF (CSF-1)/IL-34/CSF-1R pathway in the formation of macrophages in MPM, using samples from patients.

Methods

Pleural effusions (PEs), frozen tumors, primary MPM cells and MPM cell lines used in this study belong to biocollections associated with clinical databases. Cytokine expressions were studied using real-time PCR and ELISA. The Cancer Genome Atlas database was used to confirm our results on an independent cohort. An original three-dimensional (3D) coculture model including

SUBMITTER: Blondy T 

PROVIDER: S-EPMC7319783 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature