Overexpression of PGC-1α influences the mitochondrial unfolded protein response (mtUPR) induced by MPP⁺ in human SH-SY5Y neuroblastoma cells.

ABSTRACT: Parkinson's disease (PD) is a common dyskinesia disease, the mitochondrial unfolded protein response (mtUPR) may be directly or indirectly involved in the occurrence and development of PD, although the exact mechanism is unclear. We established a dopaminergic neuronal-like cell model of PD, by overexpression of PGC-1α to detect evaluate the expression of proteases and molecular chaperones of involved in the mtUPR, as well as the expression of PGC-1α and LRPPRC, illustrated the distribution of LRPPRC. Remarkably, the mtUPR activation reached maximal at 24 h after MPP⁺ treatment in SH-SY5Y cells, which the protein and transcription levels of the proteases and molecular chaperones reached maximal. The proteases and molecular chaperones were significantly increased when overexpresse

SUBMITTER: Cai Y 

PROVIDER: S-EPMC7320005 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature