Dyslexia Candidate Gene and Ciliary Gene Expression Dynamics During Human Neuronal Differentiation.

ABSTRACT: Developmental dyslexia (DD) is a neurodevelopmental condition with complex genetic mechanisms. A number of candidate genes have been identified, some of which are linked to neuronal development and migration and to ciliary functions. However, expression and regulation of these genes in human brain development and neuronal differentiation remain uncharted. Here, we used human long-term self-renewing neuroepithelial stem (lt-NES, here termed NES) cells derived from human induced pluripotent stem cells to study neuronal differentiation in vitro. We characterized gene expression changes during differentiation by using RNA sequencing and validated dynamics for selected genes by qRT-PCR. Interestingly, we found that genes related to cilia were significantly enriched among upregulated genes durin

SUBMITTER: Bieder A 

PROVIDER: S-EPMC7320047 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature