In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD⁺ fluorescent protein sensors.

ABSTRACT: The challenge of monitoring in planta dynamic changes of NADP(H) and NAD(H) redox states at the subcellular level is considered a major obstacle in plant bioenergetics studies. Here, we introduced two circularly permuted yellow fluorescent protein sensors, iNAP and SoNar, into Arabidopsis thaliana to monitor the dynamic changes in NADPH and the NADH/NAD⁺ ratio. In the light, photosynthesis and photorespiration are linked to the redox states of NAD(P)H and NAD(P) pools in several subcellular compartments connected by the malate-OAA shuttles. We show that the photosynthetic increases in stromal NADPH and NADH/NAD⁺ ratio, but not ATP, disappear when glycine decarboxylation is inhibited. These observations highlight the complex interplay between chloroplasts and mitochond

SUBMITTER: Lim SL 

PROVIDER: S-EPMC7320160 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature