Matrix metallopeptidases regulate granulosa cell remodeling through the hormone signaling pathway.

ABSTRACT:

Objective

Granulosa cells (GCs) play a very important role in reproductive physiology due to their effect on developmental and functional changes. However, there are differing views regarding the mechanism by which hormones stimulate GCs. Therefore, our study aims to determine whether GCs, in the absence of initial stimulation (17β-estradiol), select specific types of MMPs that reconstitute cells by stimulation of major hormones [follicle-stimulating hormone (FSH) or/and luteinizing hormone (LH)].

Materials and methods

Early GCs were extracted from immature follicles of the porcine ovary to analyze the MMPs levels. Using early GCs in pigs, the cell development rate was evaluated by adding 17β-estradiol, FSH, LH, or FSH + LH, respectively, to the DMEM containing 10% FBS. Real

SUBMITTER: Kim SH 

PROVIDER: S-EPMC7320815 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature