[MiR-204 inhibits invasion and metastasis of breast cancer cells by targeted regulation of HNRNPA2B1].

ABSTRACT:

Objective

To investigate the effect of miR-204 on the invasion and metastasis of breast cancer by targeted regulation of HNRNPA2B1.

Methods

The bioinformatics database was used to obtain data of the expressions of miR-204 in breast cancer patients and the survival rate of the patients. RT-qPCR was used to detect the expression of miR-204 in breast cancer cell lines. The expression vector GV369-miR-204 was used to overexpress miR-204 in MDA-MB-231 cells. Transwell assay was performed to detect the effect of miR-204 on the migration and invasion ability of the breast cancer cells. The key genes (hub genes) of miR-204 were determined by bioinformatics method. A dual luciferase assay was used to analyze the targeting relationship between miR-204 and HNRNPA2B1. The expression of

SUBMITTER: Zhang L 

PROVIDER: S-EPMC7321266 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature