LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation.

ABSTRACT: During cell division, remodelling of the nuclear envelope enables chromosome segregation by the mitotic spindle¹. The reformation of sealed nuclei requires ESCRTs (endosomal sorting complexes required for transport) and LEM2, a transmembrane ESCRT adaptor^2-4. Here we show how the ability of LEM2 to condense on microtubules governs the activation of ESCRTs and coordinated spindle disassembly. The LEM motif of LEM2 binds BAF, conferring on LEM2 an affinity for chromatin^5,6, while an adjacent low-complexity domain (LCD) promotes LEM2 phase separation. A proline-arginine-rich sequence within the LCD binds to microtubules and targets condensation of LEM2 to spindle microtubules that traverse the nascent nuclear envelope. Furthermore, the winged-helix domain of L

SUBMITTER: von Appen A 

PROVIDER: S-EPMC7321842 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature