Coupling of Ca²⁺ and voltage activation in BK channels through the αB helix/voltage sensor interface.

ABSTRACT: Large-conductance Ca²⁺ and voltage-activated K⁺ (BK) channels control membrane excitability in many cell types. BK channels are tetrameric. Each subunit is composed of a voltage sensor domain (VSD), a central pore-gate domain, and a large cytoplasmic domain (CTD) that contains the Ca²⁺ sensors. While it is known that BK channels are activated by voltage and Ca²⁺, and that voltage and Ca²⁺ activations interact, less is known about the mechanisms involved. We explore here these mechanisms by examining the gating contribution of an interface formed between the VSDs and the αB helices located at the top of the CTDs. Proline mutations in the αB helix greatly decreased voltage activation while having negligible effects on gating currents. An

SUBMITTER: Geng Y 

PROVIDER: S-EPMC7321994 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature