GPCR-dependent biasing of GIRK channel signaling dynamics by RGS6 in mouse sinoatrial nodal cells.

ABSTRACT: How G protein-coupled receptors (GPCRs) evoke specific biological outcomes while utilizing a limited array of G proteins and effectors is poorly understood, particularly in native cell systems. Here, we examined signaling evoked by muscarinic (M₂R) and adenosine (A₁R) receptor activation in the mouse sinoatrial node (SAN), the cardiac pacemaker. M₂R and A₁R activate a shared pool of cardiac G protein-gated inwardly rectifying K⁺ (GIRK) channels in SAN cells from adult mice, but A₁R-GIRK responses are smaller and slower than M₂R-GIRK responses. Recordings from mice lacking Regulator of G protein Signaling 6 (RGS6) revealed that RGS6 exerts a GPCR-dependent influence on GIRK-dependent signaling in SAN cells, suppres

SUBMITTER: Anderson A 

PROVIDER: S-EPMC7322085 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature