Midbody Remnant Inheritance Is Regulated by the ESCRT Subunit CHMP4C.

ABSTRACT: The inheritance of the midbody remnant (MBR) breaks the symmetry of the two daughter cells, with functional consequences for lumen and primary cilium formation by polarized epithelial cells, and also for development and differentiation. However, despite its importance, neither the relationship between the plasma membrane and the inherited MBR nor the mechanism of MBR inheritance is well known. Here, the analysis by correlative light and ultra-high-resolution scanning electron microscopy reveals a membranous stalk that physically connects the MBR to the apical membrane of epithelial cells. The stalk, which derives from the uncleaved side of the midbody, concentrates the ESCRT machinery. The ESCRT CHMP4C subunit enables MBR inheritance, and its depletion dramatically reduces the percentage o

SUBMITTER: Casares-Arias J 

PROVIDER: S-EPMC7322264 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature