Identification and Analysis of Natural Building Blocks for Evolution-Guided Fragment-Based Protein Design.

ABSTRACT: Natural evolution has generated an impressively diverse protein universe via duplication and recombination from a set of protein fragments that served as building blocks. The application of these concepts to the design of new proteins using subdomain-sized fragments from different folds has proven to be experimentally successful. To better understand how evolution has shaped our protein universe, we performed an all-against-all comparison of protein domains representing all naturally existing folds and identified conserved homologous protein fragments. Overall, we found more than 1000 protein fragments of various lengths among different folds through similarity network analysis. These fragments are present in very different protein environments and represent versatile building blocks for p

SUBMITTER: Ferruz N 

PROVIDER: S-EPMC7322520 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature