Two-photon excitation of FluoVolt allows improved interrogation of transmural electrophysiological function in the intact mouse heart.

ABSTRACT:

Background & aims

Two-photon excitation of voltage sensitive dyes (VSDs) can measure rapidly changing electrophysiological signals deep within intact cardiac tissue with improved three-dimensional resolution along with reduced photobleaching and photo-toxicity compared to conventional confocal microscopy. Recently, a category of VSDs has emerged which records membrane potentials by photo-induced electron transfer. FluoVolt is a novel VSD in this category which promises fast response and a 25% fractional change in fluorescence per 100 mV, making it an attractive optical probe for action potential (AP) recordings within intact cardiac tissue. The purpose of this study was to characterize the fluorescent properties of FluoVolt as well as its utility for deep tissue imaging.

Methods

SUBMITTER: Salerno S 

PROVIDER: S-EPMC7322535 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature