Conjugated linoleic acid producing potential of lactobacilli isolated from goat (AXB) rumen fluid samples.

ABSTRACT: OBJECTIVE:The present investigation was aimed to explore the potential of lactobacilli for conjugated linoleic acid (CLA) production, isolated from rumen fluid samples of lactating goats. METHODS:A total of 64 isolates of lactobacilli were obtained using deMan-Rogosa-Sharpe (MRS) agar from rumen fluid of goats and further subjected to morphological and biochemical characterizations. Isolates found as gram-positive, catalase negative rods were presumptively identified as Lactobacillus species and further confirmed by genus specific polymerase chain reaction (PCR). The phylogenetic tree was constructed from the nucleotide sequences using MEGA6. RESULTS:Out of the 64 isolates, 23 isolates were observed positive for CLA production by linoleate isomerase gene-based amplification and quantitativ

SUBMITTER: Tyagi AK 

PROVIDER: S-EPMC7322636 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature