The expression and localization of V-ATPase and cytokeratin 5 during postnatal development of the pig epididymis.

ABSTRACT: OBJECTIVE:We examined the localization and expression of H+ pumping vacuolar ATPase (V-ATPase) and cytokeratin 5 (KRT5) in the epididymis of pigs, expressed in clear and basal cells, respectively, during postnatal development. METHODS:Epididymides were obtained from pigs at 1, 7, 21, 60, 120, and 180 days of age; we observed the localization and expression patterns of V-ATPase and KRT5 in the different regions of these organs, namely, the caput, corpus, and cauda. The differentiation of epididymal epithelial cells was determined by immunofluorescence labeling using cell-type-specific markers and observed using confocal microscopy. RESULTS:At postnatal day 5 (PND5), the localization of clear cells commenced migration from the cauda toward the caput. Although at PND120, goblet-shaped clear c

SUBMITTER: Park YJ 

PROVIDER: S-EPMC7322665 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature