Reduced somatostatin signalling leads to hypersecretion of glucagon in mice fed a high-fat diet.

ABSTRACT:

Objectives

Elevated plasma glucagon is an early symptom of diabetes, occurring in subjects with impaired glucose regulation. Here, we explored alpha-cell function in female mice fed a high-fat diet (HFD).

Methods

Female mice expressing the Ca²⁺ indicator GCaMP3 specifically in alpha-cells were fed a high-fat or control (CTL) diet. We then conducted in vivo phenotyping of these mice, as well as experiments on isolated (ex vivo) islets and in the in situ perfused pancreas.

Results

In HFD-fed mice, fed plasma glucagon levels were increased and glucagon secretion from isolated islets and in the perfused mouse pancreas was also elevated. In mice fed a CTL diet, increasing glucose reduced intracellular Ca²⁺ ([Ca²⁺]_i) oscillation f

SUBMITTER: Kellard JA 

PROVIDER: S-EPMC7322681 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature