Induction of hair follicle neogenesis with cultured mouse dermal papilla cells in de novo regenerated skin tissues.

ABSTRACT: De novo skin regeneration with human keratinocytes amplified in culture is a life-saving procedure for patients with extensive skin loss and chronic wounds. It also provides a valuable platform for gene function and therapeutic assessments. Nevertheless, tissues generated in this manner lack hair follicles that are important for skin homeostasis, barrier function, and repair. In this study, we generated skin tissues with human keratinocytes combined with dermal papilla (DP) cells isolated from mouse whisker hair. For this, cultured keratinocytes and mouse DP (mDP) cells were mixed at 10:1 ratio and seeded onto devitalized human dermal matrix derived from surgically discarded human abdominoplasty skin. After 1 week in submerged culture, the cell/matrix composites were grafted onto the skin

SUBMITTER: Zhang L 

PROVIDER: S-EPMC7322722 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature