A humanized mouse model to study mast cells mediated cutaneous adverse drug reactions.

ABSTRACT: Recently a G-protein-coupled receptor, MAS Related GPR Family Member X2 (MRGPRX2), was identified as a specific receptor on human mast cells responsible for IgE independent adverse drug reactions (ADR). Although a murine homologue, Mrgprb2, has been identified for this receptor, its affinity for many ADR-causing drugs is poor making it difficult to undertake in vivo studies to examine mechanisms of ADR and to develop therapeutic strategies. Here, we have created humanized mice capable of generating MRGPRX2-expressing human MCs allowing for the study of MRGPRX2 MCs-mediated ADR in vitro as well as in vivo. Humanized mice were generated by hydrodynamic-injection of plasmids expressing human GM-CSF and IL-3 into NOD-scid IL2R-γ^-/- strain of mice that had been transplanted with huma

SUBMITTER: Mencarelli A 

PROVIDER: S-EPMC7322799 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature