Platelet-derived exosomes promote neutrophil extracellular trap formation during septic shock.

ABSTRACT:

Background

Platelets have been demonstrated to be potent activators of neutrophil extracellular trap (NET) formation during sepsis. However, the mediators and molecular pathways involved in human platelet-mediated NET generation remain poorly defined. Circulating plasma exosomes mostly originating from platelets may induce vascular apoptosis and myocardial dysfunction during sepsis; however, their role in NET formation remains unclear. This study aimed to detect whether platelet-derived exosomes could promote NET formation during septic shock and determine the potential mechanisms involved.

Methods

Polymorphonuclear neutrophils (PMNs) were cocultured with exosomes isolated from the plasma of healthy controls and septic shock patients or the supernatant of human platelets sti

SUBMITTER: Jiao Y 

PROVIDER: S-EPMC7322900 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature