Tug of War between Condensate Phases in a Minimal Macromolecular System.

ABSTRACT: Membraneless organelles, comprising dozens to hundreds of macromolecular components, form heterogeneous phases in space and evolve over time in material properties. Here, using four macromolecules, we demonstrate a range of phase behaviors associated with membraneless organelles and uncover the underlying physicochemical rules. The macromolecules are SH3₅ (S) and PRM₅ (P), two pentameric, oppositely charged protein constructs; heparin (H), an anionic polymer; and lysozyme (L), a cationic single-domain protein. The S:P, S:L, and P:H binaries form droplets, but the H:L binary forms network-like precipitates, therefore setting up a tug of war between different condensate phases within the S:P:H:L quaternary. The H:L exception can partly be attributed to the compactness o

SUBMITTER: Ghosh A 

PROVIDER: S-EPMC7323581 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature