Phenotype instability of hepatocyte-like cells produced by direct reprogramming of mesenchymal stromal cells.

ABSTRACT:

Background

Hepatocyte-like cells (iHEPs) generated by transcription factor-mediated direct reprogramming of somatic cells have been studied as potential cell sources for the development of novel therapies targeting liver diseases. The mechanisms involved in direct reprogramming, stability after long-term in vitro expansion, and safety profile of reprogrammed cells in different experimental models, however, still require further investigation.

Methods

iHEPs were generated by forced expression of Foxa2/Hnf4a in mouse mesenchymal stromal cells and characterized their phenotype stability by in vitro and in vivo analyses.

Results

The iHEPs expressed mixed hepatocyte and liver progenitor cell markers, were highly proliferative, and presented metabolic activities in function

SUBMITTER: Orge ID 

PROVIDER: S-EPMC7323614 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature