Multicomponent Microscale Biosynthesis of Unnatural Cyanobacterial Indole Alkaloids.

ABSTRACT: Genome sequencing and bioinformatics tools have facilitated the identification and expression of an increasing number of cryptic biosynthetic gene clusters (BGCs). However, functional analysis of all components of a metabolic pathway to precisely determine biocatalytic properties remains time-consuming and labor intensive. One way to speed this process involves microscale cell-free protein synthesis (CFPS) for direct gene to biochemical function analysis, which has rarely been applied to study multicomponent enzymatic systems in specialized metabolism. We sought to establish an in vitro transcription/translation (TT)-assay to assess assembly of cyanobacterial-derived hapalindole-type natural products (cNPs) because of their diverse bioactivity profiles and complex structural diversi

SUBMITTER: Khatri Y 

PROVIDER: S-EPMC7323787 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature