Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.

ABSTRACT: A number of isothermal DNA amplification technologies claim to be ideal for point-of-need (PON) applications as they enable reactions to be performed using a single-temperature heat source (e.g. water bath). Thus, we examined several isothermal amplification methods focusing on simplicity, cost, sensitivity and reproducibility to identify the most suitable method(s) for low resource PON applications. A number of methods were found unsuitable as they either involved multiple temperature incubations, were relatively expensive or required relatively large amounts target DNA for amplification. Among the methods examined, loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) were found to be the most suitable for PON applications as they are both single st

SUBMITTER: Zou Y 

PROVIDER: S-EPMC7323990 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature