Metabolites Interrogation in Cell Fate Decision of Cultured Human Corneal Endothelial Cells.

ABSTRACT: Purpose:Aiming to clarify the metabolic interrogation in cell fate decision of cultured human corneal endothelial cells (cHCECs). Methods:To analyze the metabolites in the culture supernatants (CS), 34 metabolome measurements were carried out for mature differentiated and a variety of cHCECs with cell state transition through a facility service. Integrated proteomics research for cell lysates by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed for 3 aliquots of each high-quality or low-quality cHCEC subpopulations (SP). The investigations for the focused genes involved in cHCEC metabolism were performed by using DAVID and its options "KEGG_PATHWAY." Results:The clusters of metabolites coincided well with the distinct content of CD44-/+ SPs. Both secreted pyruvic acid

SUBMITTER: Hamuro J 

PROVIDER: S-EPMC7324440 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature