Tripartite-motif family protein 35-28 regulates microglia development by preventing necrotic death of microglial precursors in zebrafish.

ABSTRACT: Microglia are tissue-resident macrophages in the central nervous system (CNS) that play essential roles in the regulation of CNS development and homeostasis. Yet, the genetic networks governing microglia development remain incompletely defined. Here, we report the identification and characterization of a microglia-defective zebrafish mutant wulong^hkz12 (wul^hkz12 ) isolated from an ethylnitrosourea (ENU)-based genetic screen. We show that wul^hkz12 mutants harbors a missense point mutation in the gene region encoding the PRY/SPRY domain of the tripartite-motif family protein 35-28 (trim35-28) gene. Time-lapse imaging revealed that the loss of Trim35-28 function causes lytic necrosis of microglial precursors/peripheral macrophages,

SUBMITTER: Yu T 

PROVIDER: S-EPMC7324517 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature