Thrombospondin-4 induces prolongation of action potential duration in rat isolated ventricular myocytes.

ABSTRACT: Expression of thrombospondin-4 (TSP-4), a matricellular protein, is increased in the heart tissue of various cardiac disease models. In dorsal root ganglion neurons, TSP-4 inhibits L-type Ca²⁺ channel (LTCC) activity. Although TSP-4 might be related to the electrophysiological properties in heart, it remains to be clarified. The present study aimed to clarify the effects of TSP-4 on action potential (AP), LTCC current (I_CaL) and voltage-dependent K⁺ (Kv) channel current (I_Kv) in rat isolated ventricular myocytes by a patch clamp technique. Ventricular myocytes were isolated from the heart of adult male Wistar rats. The ventricular myocytes were treated with TSP-4 (5 nM) or its vehicle for 4 hr. Then, whole-cell patch clamp technique was performed

SUBMITTER: Imoto K 

PROVIDER: S-EPMC7324826 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature