Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples.

ABSTRACT:

Background

COVID-19 (coronavirus disease 2019) has caused a major epidemic worldwide; however, much is yet to be known about the epidemiology and evolution of the virus partly due to the scarcity of full-length SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) genomes reported. One reason is that the challenges underneath sequencing SARS-CoV-2 directly from clinical samples have not been completely tackled, i.e., sequencing samples with low viral load often results in insufficient viral reads for analyses.

Methods

We applied a novel multiplex PCR amplicon (amplicon)-based and hybrid capture (capture)-based sequencing, as well as ultra-high-throughput metatranscriptomic (meta) sequencing in retrieving complete genomes, inter-individual and intra-individual variatio

SUBMITTER: Xiao M 

PROVIDER: S-EPMC7325194 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature