Protein Kinase A Inhibitor H89 Attenuates Experimental Proliferative Vitreoretinopathy.

ABSTRACT: Purpose:This study aimed to explore the role of the protein kinase A (PKA) pathway in proliferative vitreoretinopathy (PVR) and the effect of the PKA inhibitor H89 on experimental PVR. Methods:Epiretinal membranes (ERMs) were acquired from PVR patients and analyzed by frozen-section immunofluorescence. An in vivo model was developed by intravitreal injecting rat eyes with ARPE-19 cells and platelet-rich plasma, and changes in eye structures and vision function were observed. An in vitro epithelial-mesenchymal transition (EMT) cell model was established by stimulating ARPE-19 cells with transforming growth factor (TGF)-β. Alterations in EMT-related genes and cell function were detected. Mechanistically, PKA activation and activity were explored to assess the relationship between TGF-β1 stim

SUBMITTER: Lyu Y 

PROVIDER: S-EPMC7325625 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature