Sequence-Independent Self-Assembly of Germ Granule mRNAs into Homotypic Clusters.

ABSTRACT: mRNAs enriched in membraneless condensates provide functional compartmentalization within cells. The mechanisms that recruit transcripts to condensates are under intense study; however, how mRNAs organize once they reach a granule remains poorly understood. Here, we report on a self-sorting mechanism by which multiple mRNAs derived from the same gene assemble into discrete homotypic clusters. We demonstrate that in vivo mRNA localization to granules and self-assembly within granules are governed by different mRNA features: localization is encoded by specific RNA regions, whereas self-assembly involves the entire mRNA, does not involve sequence-specific, ordered intermolecular RNA:RNA interactions, and is thus RNA sequence independent. We propose that the ability of mRNAs to self-sort into

SUBMITTER: Trcek T 

PROVIDER: S-EPMC7325742 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature