A High-Throughput System for Cyclic Stretching of Precision-Cut Lung Slices During Acute Cigarette Smoke Extract Exposure.

ABSTRACT:

Rationale

Precision-cut lung slices (PCLSs) are a valuable tool in studying tissue responses to an acute exposure; however, cyclic stretching may be necessary to recapitulate physiologic, tidal breathing conditions.

Objectives

To develop a multi-well stretcher and characterize the PCLS response following acute exposure to cigarette smoke extract (CSE).

Methods

A 12-well stretching device was designed, built, and calibrated. PCLS were obtained from male Sprague-Dawley rats (N = 10) and assigned to one of three groups: 0% (unstretched), 5% peak-to-peak amplitude (low-stretch), and 5% peak-to-peak amplitude superimposed on 10% static stretch (high-stretch). Lung slices were cyclically stretched for 12 h with or without CSE in the media. Levels of Interleukin-1β (I

SUBMITTER: Mondonedo JR 

PROVIDER: S-EPMC7326018 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature