Systematic engineering of branch chain amino acid supply modules for the enhanced production of bacitracin from Bacillus licheniformis.

ABSTRACT: Bacitracin is a broad-spectrum cyclic peptide antibiotic mainly produced by Bacillus, precursor amino acid supply served as the critical role during its synthesis. In this study, we systematically engineered branch-chain amino acid (BCAA) supply modules for bacitracin production. Firstly, we demonstrated that Ile and Leu acted as limiting precursors for bacitracin synthesis, and that BCAA synthetic pathways were strengthened via simultaneous overexpression of, feedback-resistance acetolactate synthase IlvBN^fbr, 2-isopropylmalate synthetase LeuA^fbr and BCAA aminotransferase YbgE. Using this approach, bacitracin yield from strain DW-BCAA2 was 892.54 U/mL, an increase of 18.32% compared with that DW2 (754.32 U/mL). Secondly, the BCAA permeases, YvbW and BraB, whic

SUBMITTER: Cai D 

PROVIDER: S-EPMC7326738 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature