Dataset Information

The targeted overexpression of SlCDF4 in the fruit enhances tomato size and yield involving gibberellin signalling.

ABSTRACT: Tomato is one of the most widely cultivated vegetable crops and a model for studying fruit biology. Although several genes involved in the traits of fruit quality, development and size have been identified, little is known about the regulatory genes controlling its growth. In this study, we characterized the role of the tomato SlCDF4 gene in fruit development, a cycling DOF-type transcription factor highly expressed in fruits. The targeted overexpression of SlCDF4 gene in the fruit induced an increased yield based on a higher amount of both water and dry matter accumulated in the fruits. Accordingly, transcript levels of genes involved in water transport and cell division and expansion during the fruit enlargement phase also increased. Furthermore, the larger amount of biomass partitioned to the fruit relied on the greater sink strength of the fruits induced by the increased activity of sucrose-metabolising enzymes. Additionally, our results suggest a positive role of SlCDF4 in the gibberellin-signalling pathway through the modulation of GA₄ biosynthesis. Finally, the overexpression of SlCDF4 also promoted changes in the profile of carbon and nitrogen compounds related to fruit quality. Overall, our results unveil SlCDF4 as a new key factor controlling tomato size and composition.

SUBMITTER: Renau-Morata B

PROVIDER: S-EPMC7326986 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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The targeted overexpression of SlCDF4 in the fruit enhances tomato size and yield involving gibberellin signalling.

Renau-Morata Begoña B Carrillo Laura L Cebolla-Cornejo Jaime J Molina Rosa V RV Martí Raúl R Domínguez-Figueroa José J Vicente-Carbajosa Jesús J Medina Joaquín J Nebauer Sergio G SG

Scientific reports 20200630 1

Tomato is one of the most widely cultivated vegetable crops and a model for studying fruit biology. Although several genes involved in the traits of fruit quality, development and size have been identified, little is known about the regulatory genes controlling its growth. In this study, we characterized the role of the tomato SlCDF4 gene in fruit development, a cycling DOF-type transcription factor highly expressed in fruits. The targeted overexpression of SlCDF4 gene in the fruit induced an in ...[more]

PMID: 32606421

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Project description:Identification of tomato varieties able to exhibit higher accumulation of primary and secondary metabolites in their fruits is currently a main objective in tomato breeding. One tool to improve fruit quality is to cultivate the plants under salt stress, although improvement of fruit quality is generally accompanied by productivity losses. However, it is very interesting to implement strategies aiming at enhancing fruit quality of tomato by means of growing plants in moderate salt stress that allows for a sustainable fruit yield. The traditional tomato varieties adapted to the Mediterranean environmental constraints may be very attractive plant materials to achieve this goal, given the wide range of fruit quality traits because of their genetic diversity. Here, agronomic responses and fruit quality traits, including primary and secondary metabolites, were analyzed in fruits of two Mediterranean traditional tomato varieties named "Tomate Pimiento" ("TP") and "Muchamiel Aperado" ("MA") because of the pepper and pear shape of their fruits, using as reference the commercial cultivar "Moneymaker" ("MM"). Plants were grown without salt (control) and with moderate salt stress (50 mM NaCl), which did not affect fruit yield in any variety. "TP" is of great interest because of its high soluble solids content (SSC) in control, which is even higher in salt, whereas "MA" is very attractive because of its high Brix yield index (SSC × fruit yield), used as overall fruit quality measure. Similitude between both traditional varieties were found for primary metabolism, as they significantly increased sucrose contents compared with "MM" in red ripe fruits from plants in control and, especially, salt stress conditions. The most remarkable difference was the high constitutive levels of total amino acids in "TP" fruits, including the three major free amino acids found in tomato fruit, GABA, glutamate, and glutamine, which even increased under salinity. Regarding secondary metabolites, the most interesting change induced by salinity was the increase in α-tocopherol found in red ripe fruits of both "TP" and "MA." These results reveal the interest of traditional varieties as sources of genetic variation in breeding because of their improvement of tomato fruit quality without production losses under moderate salt stress.

Project description:BackgroundFruit maturation and ripening are genetically regulated processes that involve a complex interplay of plant hormones, growth regulators and multiple biological and environmental factors. Tomato (Solanum lycopersicum) has been used as a model of biological and genetic studies on the regulation of specific ripening pathways, including ethylene, carotenoid and cell wall metabolism. This model has also been used to investigate the functions of upstream signalling and transcriptional regulators. Thus far, many ripening-associated transcription factors that influence fruit development and ripening have been reported. NAC transcription factors are plant specific and play important roles in many stages of plant growth and development, such as lateral root formation, secondary cell wall synthesis, and embryo, floral organ, vegetative organ and fruit development.ResultsTissue-specific analysis by quantitative real-time PCR showed that SlNAC1 was highly accumulated in immature green fruits; the expression of SlNAC1 increased with fruit ripening till to the highest level at 7 d after the breaker stage. The overexpression of SlNAC1 resulted in reduced carotenoids by altering carotenoid pathway flux and decreasing ethylene synthesis mediated mainly by the reduced expression of ethylene biosynthetic genes of system-2, thus led to yellow or orange mature fruits. The results of yeast one-hybrid experiment demonstrated that SlNAC1 can interact with the regulatory regions of genes related lycopene and ethylene synthesis. These results also indicated that SlNAC1 inhibited fruit ripening by affecting ethylene synthesis and carotenoid accumulation in SlNAC1 overexpression lines. In addition, the overexpression of SlNAC1 reduced the firmness of the fruits and the thickness of the pericarp and produced more abscisic acid, resulting in the early softening of fruits. Hence, in SlNAC1 overexpression lines, both ethylene-dependent and abscisic acid-dependent pathways are regulated by SlNAC1 in fruit ripening regulatory network.ConclusionsSlNAC1 had a broad influence on tomato fruit ripening and regulated SlNAC1 overexpression tomato fruit ripening through both ethylene-dependent and abscisic acid-dependent pathways. Thus, this study provided new insights into the current model of tomato fruit ripening regulatory network.

Project description:In regions with intensive agricultural production, large amounts of organic waste are produced by livestock animals. Liquid digestate from manure-based biogas production could potentially serve as fertilizer if integrated with closed horticultural irrigation systems. The aim of this experiment was to investigate how fertilizer based on liquid biogas by-products of pig manure digestion can affect the growth and production of tomato plants. Integration of a nitrification bioreactor presumes a significantly lower concentration of nutrient solutions and a higher level of oxygenation than classical mineral cultivation. Therefore, additional controls were included. We compared plant growth and fruit quality traits of tomato plants grown in a hydroponic solution with organic fertilizer with two levels of mineral fertilizer. The tomatoes grown with organic waste-based liquid fertilizer showed reduced growth rates but increased mean fruit size, resulting in no significant change in total yield compared with high-mineral cultivation. The growth rate was similarly reduced in plants cultivated with low-mineral fertilizer. Plants cultivated with organic waste-based fertilizer had high Cl- concentration in xylem sap, leaves, and, ultimately, fruits. The leaves of plants cultivated with organic waste-based fertilizer contained higher concentrations of starch and soluble carbohydrate and low concentrations of phosphorous (P) and sulfur (S). The plants grown with organic waste-based or low-mineral medium showed significantly poorer fruit quality than the plants cultivated with the high-mineral solution. The low-mineral treatment increased xylem sap contribution to fruit weight because of higher root power. The organic waste-based fertilization did not change the root power but increased fruit size. In conclusion, organic waste-based cultivation is a possible solution for sustainable plant production in greenhouses. However, additional adjustment of nutrient supply is required to improve fruit quality.

Dataset Information

The targeted overexpression of SlCDF4 in the fruit enhances tomato size and yield involving gibberellin signalling.

Publications

The targeted overexpression of SlCDF4 in the fruit enhances tomato size and yield involving gibberellin signalling.

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